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Rice, 1 Ed.
出版済み 3週間でお届けいたします。
Methods and Protocols Series: Methods in Molecular Biology, Vol. 2869 Author: Taiji Kawakatsu Publisher: Springer ISBN: 9781071642030 Cover: HARDCOVER Date: 2024年11月 DESCRIPTION This volume aims to be a comprehensive guide to the field of rice molecular biology, encompassing both time-honored techniques and state-of-the-art methodologies. Chapters detail sample preparation, temporal and spatial analysis of RNA, protein expression, transformation and precise genome editing, scalable transcriptome analysis, epigenome analysis, and gene regulatory network analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Rice: Methods and Protocols aims to ensure successful results in the further study of this vital field. TABLE OF CONTENTS Hydroponic Culture of Rice Seedlings for Stress Response Assay Fumiyuki Soma, Yusaku Uga Total RNA Extraction from Rice Vegetative Tissues Using Magnetic Beads Kanami Yoshino, Taiji Kawakatsu Total RNA Extraction from Starchy Rice Endosperm Yuhya Wakasa RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection Takumi Tezuka, Misuzu Nosaka-Takahashi, Takanori Yoshikawa, Yutaka Sato Using Immunoblotting for Detection of a Circadian Clock Component, Os-GI, in Rice Leaves Hironori Itoh Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry Masayuki Miya, Ken-ichiro Hibara, Jun-ichi Itoh Spatial Analysis of Gene Expression by In Situ Hybridization Masayuki Miya, Ken-ichiro Hibara, Jun-ichi Itoh Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem Takumi Tezuka, Misuzu Nosaka-Takahashi, Takanori Yoshikawa, Yutaka Sato Agrobacterium-Mediated Transformation and Targeted Mutagenesis Using SpCas12f in Rice Satoru Sukegawa, Seiichi Toki, Hiroaki Saika Gene Targeting via CRISPR/Cas9-Mediated DNA Double-Strand Break Induction in Rice Ayako Nishizawa-Yokoi, Seiichi Toki Precise Base Substitution Using CRISPR/Cas-Mediated Base Editor in Rice Masaki Endo, Katsuya Negishi, Seiichi Toki DeLTa-Seq: High-Throughput Targeted RNA-Seq of Rice Leaves Without RNA Purification Makoto Kashima, Yasuyuki Nomura, Atsushi J. Nagano Lasy-Seq: A High-Throughput 3′ RNA-Seq Method for Large-Scale Transcriptome Profiling in Rice Natsumi Mori-Moriyama, Atsushi J. Nagano Profiling of 24-nt phasiRNAs in Rice Anther by Massively Parallel Sequencing and Stem-Loop Reverse Transcription PCR Huong Ta, Ken-Ichi Nonomura Expression and Purification of pAG-MNase for CUT&RUN Taiji Kawakatsu Chromatin Immunoprecipitation from Shoot Apices and Young Panicles of Rice Katsutoshi Tsuda In Vitro Genome-Wide Identification of Transcription Factor Binding Sites Taiji Kawakatsu High-Efficient Transient Gene Transfection for Transcriptional Activity Assay Using Rice Protoplasts on Large-Scale Platform Shingo Sakamoto
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