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Gene Cloning and DNA Analysis, 9 ed
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Title: Gene Cloning and DNA Analysis, 9 ed Subtitle: An Introduction Author: Brown, T. A. (University of Manchester, UK) Publisher: Wiley ISBN: 9781394292561 Cover: PAPERBACK Date: 2025年11月 DESCRIPTION New edition of a world-renowned introductory text to the rapidly-growing and dynamic field of Gene Cloning and DNA Analysis Gene Cloning and DNA Analysis is a comprehensive introductory text that explores all of the topics vital for a complete understanding of gene cloning and DNA analysis. Presented in full color, the text’s easy-to-follow layout and over 250 clear illustrations make the material simple and accessible for a diverse readership. This newly revised and updated Ninth Edition highlights new developments in DNA sequencing technology and provides extended coverage on DNA editing including CRISPR methodology and its applications in gene therapy and plant genetic engineering. This edition also contains updates on topics including optical mapping of DNA molecules, studying transcriptomes in situ, RNA interference methods for silencing genes in crop plants, studying kinship by DNA profiling, and using ancient DNA to study human prehistory and palaeogenomics. Gene Cloning and DNA Analysis also discusses topics such as: * The strategies used by researchers and industry practitioners to assemble telomere-to-telomere chromosome sequences * The synthesis of recombinant vaccines for diseases such as COVID-19 and hepatitis B * Interbreeding between Neanderthals and Homo sapiens * The ethical issues raised by the use of gene cloning and DNA editing in pharming, gene therapy and plant genetic modification The Ninth Edition of Gene Cloning and DNA Analysis continues to be an essential reference for undergraduate and graduate students within the fields of genetics and genomics, molecular biology, biochemistry, immunology, and applied biology, as well as professionals in all areas of biological science. TABLE OF CONTENTS Part I The Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis Are Important? 2 Vectors for Gene Cloning: Plasmids and Bacteriophages 3 Purification of DNA from Living Cells 4 Manipulation of Purified DNA 5 Introduction of DNA into Living Cells 6 Cloning Vectors for Escherichia coli 7 Cloning Vectors for Eukaryotes 8 How to Obtain a Clone of a Specific Gene 9 The Polymerase Chain Reaction Part II The Applications of Gene Cloning and DNA Analysis in Research 10 Sequencing Genes and Genomes 11 Studying Gene Expression and Function 12 Studying Genomes 13 Studying Transcriptomes and Proteomes 14 DNA Editing Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 15 Production of Protein from Cloned Genes 16 Gene Cloning and DNA Analysis in Medicine 17 Gene Cloning and DNA Analysis in Agriculture 18 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 最近チェックした商品
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