Plant Cell Culture Protocols, 5 Ed.
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Series: Methods in Molecular Biology Author: Neftali Ochoa-Alejo; Victor Loyola-Vargas Publisher: Springer ISBN: 9781071639535 Cover: HARDCOVER Date: 2024年07月 DESCRIPTION This fifth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Cell Culture Protocols, Fifth Edition aims to ensure successful results in the further study of this vital field. TABLE OF CONTENTS An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives Victor M. Loyola-Vargas, Neftali Ochoa-Alejo Use of Statistics in Plant Cell Tissue Culture Michael E. Compton Plant Micropropagation and Temporary Immersion Systems Hugo A. Mendez-Hernandez, Victor M. Loyola-Vargas The History of Agrobacterium Rhizogenes: From Pathogen to a Multitasking Platform for Biotechnology Victor M. Loyola-Vargas, Hugo A. Mendez-Hernandez, Ana O. Quintana-Escobar Use of Electron Microscopy for the Detection of Contaminant Endophytic Bacteria In Vitro Inae Marie de Araujo Silva-Cardoso, Mariana Oliveira Medeiros, Ana Cristina Meneses Mendes Gomes, Andre Luis Xavier de Souza, Jonny Everson Scherwinski-Pereira Using Mathematical Optimization Models to Improve the Efficiency of Duckweeds (Wolffia arrhiza and Lemna minor) Micropropagation Pavel Khvatkov, Sergey Dolgov Application of Artificial Neural Network and Genetic Algorithm Modeling for In Vitro Regeneration of Seaweed Seedling Production Ramalingam Dineshkumar, Mudassar Anisoddin Kazi, Vaibhav A. Mantri Less Frequently Used Growth Regulators in Plant Tissue Culture Sergio J. Ochatt Protocol for Generating Arabidopsis thaliana Cell Suspension Cultures Under Different Light Conditions Sara Hernandez-Castellano, Rosa Ma. Galaz-Avalos, Victor M. Loyola-Vargas, Clelia De-la-Pena Micropropagation of Agave angustifolia “Espadin” Used in the Production of Mezcal Gabriel Ojeda, Antonio Rescalvo-Morales, Kelly Maribel Monja-Mio, Lorenzo Felipe Sanchez-Teyer In Vitro Propagation of Threatened Agave Species Using a Two-Stage System Lucia Isabel Chavez Ortiz, Eugenio Perez Molphe Balch In Vitro Propagation of “Salsaparrilha” [Herreria salsaparrilha Mart. (Asparagaceae)]: An Antisyphilitic Medicinal Species Leticia Almeida Goncalves, Robson Maia Geraldine, Sergio Heitor Sousa Felipe, Elisandra Silva Sousa, Juliane Maciel Henschel, Diego Silva Batista et al. Micropropagation of the Ornamental Aquatic Plant, Aponogeton ulvaceus, from Immature Tuber Explants Melissa Yit Yee Kam, Chiew Foan Chin Protocol for the Acclimatization of Coconut Vitro-Plants Under Ex Vitro Conditions Maria Narvaez, Natali Gomez-Falcon, Carlos Oropeza Advances in Tissue Culture and Transformation Studies in Non-model Species: Passiflora spp. (Passifloraceae) Wagner Campos Otoni, Jessica Ribeiro Soares, Claudinei Silva Souza, Lazara Aline Simoes Silva, Lana Laene Lima Dias, Kerly Jessenia Moncaleano Robledo et al. Advances in Tissue Culture and Transformation Studies in Non-model Species: Bixa orellana L. (Bixaceae) Wagner Campos Otoni, Kleiton Lima Godoy Machado, Ludmila Nayara de Freitas Correia, Elyabe Monteiro Matos, Elisandra Silva Sousa, Lazara Aline Simoes Silva et al. Anther Culture Protocols for Barley and Wheat Sue Broughton, Marieclaire Castello, Li Liu, Julie Killen, Christopher McMullan Synthetic Tetraploid of Oncidium crispum Lodd. (Orchidaceae) Thalita Bordignon da Cunha, Saulo Marcal de Sousa, Aryane Campos Reis, Elyabe Monteiro de Matos, Lyderson Facio Viccini Direct Somatic Embryogenesis in Carica papaya L. Genotypes for Genetic Modification Purposes Amaranta Giron-Ramirez, Yessica Bautista-Bautista, Humberto Estrella-Maldonado, Arianna Chan-Leon, Eddy Turrent, Carlos Talavera et al. Scale-Up of Coffea canephora Somatic Embryogenesis in Temporary Immersion System Hugo A. Mendez-Hernandez, Victor M. Loyola-Vargas Large-Scale Production of Specialized Metabolites In Vitro Cultures Andrey S. Marchev, Iva D. Stoykova, Milen I. Georgiev Quantification of DNA Methylation by ELISA in Epigenetic Studies in Plant Tissue Culture: A Theoretical-Practical Guide Marilia Santos Silva Patriota, Regina Beatriz Bernd, Andre Luis Xavier de Souza, Luis Alberto Martins Palhares de Melo, Jonny Everson Scherwinski-Pereira Proteomic Analysis of In Vitro Plant Tissues (Coffea canephora): A Case of Study Ana O. Quintana-Escobar, Victor M. Loyola-Vargas Transcriptomic Analysis During the Induction of Somatic Embryogenesis in Coffea canephora Ana O. Quintana-Escobar, Victor M. Loyola-Vargas Chloroplast Isolation in Agavaceas Luis F. Nunez-Becerril, Sara Hernandez-Castellano, Veronica Limones-Briones, Clelia De-la-Pena Overexpression of Genes to Enhance Abiotic Stress Tolerance in Arabidopsis Mahmoud W. Yaish Plant Tissue Culture and Metabolite Profiling for High-Value Natural Product Synthesis Youjun Zhang, Kezhen Qin, Alisdair R. Fernie A Novel Method for In Situ RT-PCR Based on Capsules from Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues Jose Ignacio Laines-Hidalgo, Angela F. Ku-Gonzalez, Felipe A. Vazquez-Flota Isolation of Protoplasts from Tissues of Mexican Prickly Poppy (Argemone mexicana L.): An Alkaloid-Producing Medicinal Plant Maria Fernanda De la Cruz-Velueta, Jose Armando Munoz-Sanchez, Felipe A. Vazquez-Flota
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