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Neuronal Morphogenesis
出版済み 3-5週間でお届けいたします。
Methods and Protocols Series: Methods in Molecular Biology Author: Kazuhito Toyooka Publisher: Humana Press ISBN: 9781071639689 Cover: HARDCOVER Date: 2024年08月 DESCRIPTION This volume details molecular mechanisms that are involved in neuronal morphogenesis, and methods for analyzing these mechanisms. Chapters detail analyzing neuronal morphology in vitro and in vivo using a variety of model systems, including rodents and human induced pluripotent stem cells (hiPSCs). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neuronal Morphogenesis: Methods and Protocols aims to be suitable for a wide range of readers interested in the mechanisms of neuronal morphology and related neurological disorders. TABLE OF CONTENTS Culturing Primary Cortical Neurons for Live-Imaging Kyle R. Northington, Emily Anne Bates Electron Microscopy of Neurons on Biomimetic Substrates Claudia Latte Bovio, Valentina Mollo, Anna Mariano, Francesca Santoro Analysis of Neurite and Spine Formation in Neurons In Vitro Jenny R. Diaz, Martin J. Sadowski Quantifying Dendritic Arbors In Vitro and In Vivo in Rodent Models Rebecca J. Wilson, Jessie R. Badley, Pamela J. Lein Visualizing and Measuring Dendrite Arborization in Drosophila Somatosensory Neurons Yineng Xu, Inle Bush, Chun Han Analyses of Neurite and Spine Formation by In Utero Electroporation in Mice Hidenori Tabata, Nanako Hamada, Koh-ichi Nagata Analysis of Neuronal Morphology by Two-Photon Microscopy Chia-Wei Huang, Yen-Lin Su, Jin-Wu Tsai In Ovo Electroporation of Embryonic Chicken Spinal Cord in Combination with Ex Vivo Slice Culture for Live Imaging of Vertebrate Neuronal Differentiation Victoria E. Higgs, Gabriela Toro-Tapia, Holly B. Burbidge, Raman M. Das In Vitro and In Vivo Analysis of Neuronal Polarity Mini Jose Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation Kanika Sharma, Prashant Kaushal, Vikas Kumar 3D Reconstruction of the Mitochondrial Network within the Neuronal Soma from SBF-SEM Volume Data Jane Tweedy, Ross Laws, George Merces, Tracey Davey, Amy K. Reeve, Amy E. Vincent DNeuroMAT: A Deep-Learning-Based Neuron Morphology Analysis Toolbox Min Liu, Zhuangdian Lin, Weixun Chen, Erik Meijering, Yaonan Wang Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation Xiaonan Liu, Kazuhito Toyo-oka Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy Takeo Saneyoshi, Yasunori Hayashi Visualization and Quantification of Organelle Axonal Transport in Cultured Neurons Jayne Aiken, Erika L. F. Holzbaur Pages 219-234 Analysis of Microtubule Polymerization During Axon Outgrowth Using Fluorescently Labeled Microtubule Plus-End Tracking Proteins Isabel Perez-Ferrer, Carlos Sanchez-Huertas Interrogating the Molecular Clutch in Neuronal Growth Cones: Measuring Traction Forces, F-actin Retrograde Flow, and Point Contact Demographics Ketakee Ghate, Sampada P. Mutalik, Aurnab Ghose Measuring Retrograde Actin Flow in Neuronal Growth Cones Laura Pulido Cifuentes, Daniel M. Suter Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers Osvaldo A. Miranda, Giselle Cheung, Simon Hippenmeyer Isolation and Culture of Adult Rat Dorsal Root Ganglion Neurons for the In Vitro Analysis of Peripheral Nerve Degeneration and Regeneration Kazunori Sango, Shizuka Takaku, Naoko Niimi, Hideji Yako A Live Imaging Assay for Regenerating Peripheral Neurons Alice E. Mortimer, Raman M. Das, Adam J. Reid Nerve Regeneration Using Compartmentalized System in CIPN Model Prashant Adhikari, Khayzaran Qubbaj, In Hong Yang Live Imaging Analysis of Axonal Regeneration in Human iPSC-Derived Motor Neurons Using a Microfluidic System Katherine L. Marshall, Mohamed H. Farah In Vivo Neural Tract Tracing Procedures: Unravelling Neural Hodology Using Fluorescent and Non-fluorescent Neural Tract Tracers, Optogenetic Approach, and Diffusion Tensor Neurotractography Protocols Norberto Cysne Coimbra, Paloma Molina Hernandes, Davi Henrique Salvador Pereira dos Santos, Maria de Fatima dos Santos Sampaio, Renato Leonardo de Freitas, Farhad Ullah et al.
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