Seed Dormancy, 1 Ed.
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Methods and Protocols Series: Methods in Molecular Biology, Vol. 2830 Author: Naoto Kawakami; Kazuhiro Sato Publisher: Springer ISBN: 9781071639641 Cover: HARDCOVER Date: 2024年07月 DESCRIPTION This volume provides basic and state-of-the-art methods for fundamental seed research and practical application. Chapters detail recent seed dormancy studies, basic methods for germination and longevity, seed imaging, sugar, chromatin analyses, QTL analysis, and GWAS protocols for diploid and complex hexaploid species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Seed Dormancy: Methods and Protocols aims to ensure successful results in the further study of this vital field. TABLE OF CONTENTS Introduction Genetic, Epigenetic, and Environmental Control of Seed Dormancy and Germination Masahiko Otani, Lipeng Zheng, Naoto Kawakami Regulation of Seed Dormancy Genes in Triticeae Species Kazuhiro Sato, Shingo Nakamura, Masaya Fujita Dormancy, Germination and Longevity Assays Assessment of Dormancy Level and Germination Ability of Seeds with Physiological Dormancy Lipeng Zheng, Masahiko Otani, Naoto Kawakami Germination Assays for Comparable Seed Dormancy Depth and Distinction of Seed Color by Multiplex PCR in Wheat Eiko Himi, Shiho Kurihara-Yonemoto Assessing Seed Germination Response of Parasitic Plant Striga hermonthica with Small-Molecule Probes Jia Xin Yap, Yuichiro Tsuchiya A Seed Storage Protocol to Determine Longevity Julia Buitink, Olivier Leprince Biochemical, Molecular Biological and Morphological Analyses Analysis of Raffinose Metabolism-Related Sugar Components in Seeds Tao Li, Tianyong Zhao Chromatin Immunoprecipitation Using Imbibed Seeds of Arabidopsis thaliana Yongmin Kwon, Giltsu Choi Confocal Imaging of Seeds Dennys-Marcela Gomez-Paez, Enrico Magnani Genetic Analyses and Gene Manipulation Map-Based Cloning of the Causal Gene for a Seed Dormancy Quantitative Trait Locus in Barley Shingo Nakamura, Kazuhiro Sato Genome-Wide Association Study of Preharvest Sprouting in Wheat Jian Yang, Jirui Wang Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat Fumitaka Abe, Masahiko Mori, Shingo Nakamura Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat Fumitaka Abe, Yoko Kamiya, Yuji Ishida, Hiroshi Hisano, Kanako Kawaura, Toshihiko Komari et al. Targeted Modification of Grain Dormancy Genes in Barley Hiroshi Hisano, Robert E. Hoffie, Jochen Kumlehn, Kazuhiro Sato In Planta Genome Editing in Commercial Wheat Varieties: Use of TaQsd1 to Lengthen Seed Dormancy Weifeng Luo, Yuelin Liu, Ryozo Imai Breeding, Practical Applications Evaluation Methods for Preharvest Sprouting Resistance in Japanese Wheat Breeding Programs Masaya Fujita, Nana Ashikaga, Hitoshi Matsunaka, Makiko Chono, Shingo Nakamura
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