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Manual of Molecular Microbiology
出版済み 3-5週間でお届けいたします。
Title: Manual of Molecular Microbiology Subtitle: Fundamentals and Applications Series: ASM Books Author: Hayden, Randall T. (St. Jude Children's Research Hospital, Memphis, TN, USA) / Carroll, Karen C. (Johns Hopkins University School of Medicine, Baltimore, MD, USA) / Dekker, John P. (National Institutes of Health, Bethesda, MD, USA) / McAdam, Alexander J. Publisher: American Society for Microbiology ISBN: 9781683674566 Cover: HARDCOVER Date: 2025年06月 DESCRIPTION Your essential guide to design, operation, management, and health care integration of the modern molecular microbiology laboratory This comprehensive resource offers definitive guidance on the operational and interpretive aspects of clinical molecular microbiology. Tailored for medical laboratory professionals, it provides practical “how-to” guidance for establishing, maintaining, and advancing molecular microbiology testing services and details the unique expertise required to support infectious disease diagnostics. The Manual offers a clear and practical roadmap for topics ranging from selecting appropriate technologies, instruments, and analytic pipelines to navigating complex interpretive challenges and positioning diagnostic testing services for future clinical and population health needs. Beginning with foundational technologies and their clinical applications, this book offers accessible overviews of each method’s potential, implications, and emerging roles. Subsequent sections dive meticulously into details of laboratory setup, design, and operations, empowering readers with hands-on insights for routine and advanced testing methods, including advanced sequencing technologies. It also tackles the nuanced challenges of interpreting and reporting results from cutting-edge diagnostics, including those focused on antimicrobial resistance and metagenomics. The final section explores the broader impact of molecular microbiology on value-based care, with discussions on clinical management, laboratory stewardship, and the future of molecular diagnostics in public health. Comprehensive and forward-looking, the Manual of Molecular Microbiology equips readers with both foundational knowledge and practical expertise, making it an indispensable reference for today’s clinical laboratory professionals. TABLE OF CONTENTS Section I Technologies and Clinical Applications 1 Real-Time PCR Jocelyn Hauser, Eleanor Powell, And N. Esther Babady 2 Digital PCR Alexandra S. Whale, Denise M. O’sullivan, And Jim F. Huggett 3 Isothermal Nucleic Acid Amplification Technologies and CRISPR-Cas-Based Nucleic Acid Detection Strategies for Infectious Diseases Diagnostics Benjamin M. Liu 4 Strain Typing (Bacterial, Viral, Fungal, and Mycobacterial) Helena M.b. Seth-smith, Ashley M. Rooney, And Adrian Egli 5 Antimicrobial Resistance Genotyping Heba H. Mostafa and Patricia J. Simner 6 Broad-Range Diagnostic Amplification and Detection Oyvind Kommedal and Ruben Dyrhovden 7 Characterization of the Human Microbiome in Health and Disease Chin Yee Tan and Neeraj K. Surana 8 Host Transcriptomics: Clinical Applications Asuncion Mejias and Octavio Ramilo 9 Organism Transcriptomics Aryeh Solomon and Roi Avraham Section II Design, Implementation, and Operations: General 10 Laboratory Design for Molecular Technologies Erin Graf 11 Sample Selection, Preservation, Transport, and Storage James J. Dunn and Erin H. Graf 12 Nucleic Acid Extraction and Purity, Including Performance Considerations of Different Extraction Chemistries Bijal A. Parikh and Neil W. Anderson 13 Selection of Nucleic AcidAmplification Systems: In Vitro Diagnostic Tests Cecilia M. Thompson, Heba H. Mostafa, And Kyle G. Rodino 14 Selection of Nucleic Acid Amplification Systems: Laboratory Developed Tests Meghan W. Starolis 15 Verification, Validation, and Quality Assurance for Nucleic Acid Amplification Tests Paige M.k. Larkin, Ninad Mehta, And Rebekah M. Martin 16 Molecular Microbiology Standardization Jerry Boonyaratanakornkit Section III Design, Implementation, and Operations: Sequencing-Based Technologies 17 Sequencing Platforms Hege Vangstein Aamot, Jacob Moran-Gilad, and John W. A. Rossen 18 Nucleic Acid Extraction and Next-Generation Sequencing Library Prep Abraham G. Moller, David C. Gaston, And Augusto Dulanto Chiang 19 Considerations of Depth, Coverage, and Other Read Quality Metrics Derek D. N. Smith and Andrea D. Tyler 20 Pipelines and Databases-Genome Assembly and Analysis from Bacterial Isolates Natalie Scherff, Hauke Tonnies, Gabriel Elias Wagner, and Alexander Mellmann 21 Analysis of Metagenomic Next-Generation Sequencing Data Obtained from Clinical Samples Brian O’donovan, Steve Miller, And Katrina Kalantar 22 Pipelines and Databases- Microbiome Analysis Jonathan Sanders, Huang Lin, Qiyun Zhu, And James T. Morton 23 Validation and Quality Control of Next-Generation Sequencing Assays Robert Schlaberg 24 Application-Specific Considerations for Clinical and Epidemiologic Sequencing Lydia A. Krasilnikova, Mohamad R. A. Sater, and Melis N. Anahtar 25 Generation and Analysis of Host Transcriptomics and Development of Host-Response Based Signatures Emily C. Lydon, Katrina L. Kalantar, And Charles R. Langelier Section IV Special Challenges in Result Interpretation and Reporting 26 Bacterial Antimicrobial Susceptibility Testing Romney Humphries 27 Public Health Surveillance Allen Bateman and Sara J. Blosser 28 Considerations in Molecular Strain Typing for Bacteria, Fungi, and Viruses S. Wesley Long 29 Considerations in Result Interpretation and Reporting for Untargeted and Broadly Targeted Pathogen Detection Sequencing Assays Heather L. Glasgow and Steve Miller 30 Considerations in Result Interpretation and Reporting for Microbiome Analysis Arryn Craney 31 Clinical Interpretation of Ultrasensitive Molecular Assays Daniel A. Ortiz and Blake W. Buchan Section V Molecular Microbiology’s Impact on Value-based Care 32 Clinical Interpretation and Diagnostic Management Swathi Gowtham 33 The Role of Clinical Microbiology in Antimicrobial and Diagnostic Stewardship Amy J. Mathers 34 Evidence-Based Assay Deployment- Support of Population Health Initiatives Susan M. Benson 35 Evidence-Based Assay Deployment- Precision Medicine Initiatives Julie Hirschhorn and Haluk Kavus 36 Evidence-Based Assay Deployment- Infrastructure, Documentation, and Strategy Natalie N. Whitfield and Donna M. Wolk
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